Chloramphenicol ELISA test Kit
Catalog No. LSY-10007
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 25℃
Incubation Time: 30min～30min～15min
Detection limit:
Egg, milk (Method 1) 0.1 ppb
Urine, serum, intestine, feed, milk powder 0.05 ppb
Tissue, liver, honey, milk (Method 2) 0.025 ppb
Recovery rate
Tissue, liver 85±20%
Honey, intestine 83±25%
Milk, feed 75±23%
Urine, serum…………………………………………………………………70±18%
Cross-reaction rate:
Chloramphenicol 100%
Thiamphenicol < 0.1%
Florfeniol < 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3） Enzyme conjugate (12 mL) red cap
4） Concentrated antibody working solution (1 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap